Whole community transcriptome of a sequencing batch reactor transforming 2,4-dinitroanisole (DNAN) and 3-nitro-1,2,4-triazol-5-one (NTO).
Biodegradation
; 29(1): 71-88, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-29143903
ABSTRACT
Two sequencing batch reactors (SBRs) were run to bio-mineralize 2,4-dinitroanisole (DNAN) and 3-nitro-1,2,4-triazol-5-one (NTO) in lab scale settings. The reactors were shown to reproducibly biotransform these munitions under aerobic and anaerobic conditions during the operations of these SBRs. Complete removal (100% biotransformation) of DNAN (initially 17.7 ± 5.4 mg L-1) and NTO (initially 15.0 ± 7.1 mg L-1) was observed in an anaerobic SBR when Luria-Bertani (LB) broth was present. In contrast, an aerobic SBR degraded only 58 ± 22% of DNAN (initially 19.7 ± 6.2 mg L-1) and 45 ± 24% of NTO (initially 9.7 ± 6.3 mg L-1) when either LB or glucose was also added indicating that anaerobic conditions are more favorable for biotransformation of these munitions. Transcriptomic analysis of the DNAN and NTO degrading anaerobic SBR revealed upregulation of a putative nitroreductase, hydroxylaminophenol mutases, 4-hydroxylphenyl acetate associated genes, and quinone oxioreductases. Major Bacterial populations included Bacteroidales, Campylobacterales, Enterobacteriales, Pseudomonadales, Burkholderiales and Clostridiales. Results from this study can be used to inform investigation of munition degrading organisms and the functional genes responsible.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Triazóis
/
Reatores Biológicos
/
Técnicas de Cultura Celular por Lotes
/
Transcriptoma
/
Anisóis
/
Nitrocompostos
Idioma:
En
Revista:
Biodegradation
Assunto da revista:
BIOQUIMICA
/
SAUDE AMBIENTAL
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS