A highly efficient Agrobacterium tumefaciens-mediated transformation system for the postharvest pathogen Penicillium digitatum using DsRed and GFP to visualize citrus host colonization.

Vu, Tao Xuan; Ngo, Tho Tien; Mai, Linh Thi Dam; Bui, Tri-Thuc; Le, Diep Hong; Bui, Ha Thi Viet; Nguyen, Huy Quang; Ngo, Binh Xuan; Tran, Van-Tuan

Vu, Tao Xuan; Ngo, Tho Tien; Mai, Linh Thi Dam; Bui, Tri-Thuc; Le, Diep Hong; Bui, Ha Thi Viet; Nguyen, Huy Quang; Ngo, Binh Xuan; Tran, Van-Tuan.

Afiliação

Vu TX; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Ngo TT; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Mai LTD; Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Bui TT; Faculty of Biotechnology and Food Technology, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen, Viet Nam.
Le DH; Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Bui HTV; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Nguyen HQ; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.
Ngo BX; Faculty of Biotechnology and Food Technology, Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen, Viet Nam.
Tran VT; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam. Electronic address: tuantran@vnu.edu.vn.

J Microbiol Methods ; 144: 134-144, 2018 01.

Article em En | MEDLINE | ID: mdl-29175534

Assuntos

Agrobacterium tumefaciens/genética; Citrus/microbiologia; Técnicas de Transferência de Genes; Genética Microbiana/métodos; Proteínas de Fluorescência Verde/genética; Proteínas Luminescentes/genética; Penicillium/genética; Cinamatos/farmacologia; DNA Bacteriano; Proteínas Fúngicas/genética; Proteínas Fúngicas/metabolismo; Regulação Fúngica da Expressão Gênica; Vetores Genéticos; Proteínas de Fluorescência Verde/metabolismo; Interações Hospedeiro-Patógeno; Humanos; Higromicina B/análogos & derivados; Higromicina B/farmacologia; Proteínas Luminescentes/metabolismo; Testes de Sensibilidade Microbiana; Mutagênese Insercional; Penicillium/efeitos dos fármacos; Penicillium/isolamento & purificação; Doenças das Plantas/microbiologia; Estreptotricinas/farmacologia; Transformação Genética; Vietnã

Palavras-chave

Agrobacterium tumefaciens-mediated transformation; Citrus host colonization; Fluorescent reporter genes; Insertional mutagenesis; Penicillium digitatum; Postharvest pathogen

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicillium / Citrus / Agrobacterium tumefaciens / Técnicas de Transferência de Genes / Proteínas de Fluorescência Verde / Genética Microbiana / Proteínas Luminescentes Limite: Humans País/Região como assunto: Asia Idioma: En Revista: J Microbiol Methods Ano de publicação: 2018 Tipo de documento: Article País de publicação: Holanda

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