TAT-conjugated chitosan cationic micelle for nuclear-targeted drug and gene co-delivery.
Colloids Surf B Biointerfaces
; 162: 326-334, 2018 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-29223647
ABSTRACT
We developed a high-efficiency nucleus-targeted co-delivery vector that delivers genes and drugs directly into the nucleus of cancer cells. The system is based on grafted poly-(N-3-carbobenzyloxy-lysine) (CPCL) with transactivator of transcription (TAT)- chitosan on the surface. It is designed to perform highly efficient nucleus- targeted gene and drug co-delivery. Confocal laser scanning microscopy (CLSM) revealed that more TAT-CPCL entered the nucleus than does CPCL alone. The TAT-modified vector serves as a gene and drug co-delivery mechanism to achieve high gene transfection efficiency, high apoptosis and low viability in HeLa cells. TAT-CPCL may become a vector for cancer gene treatment and a template for designing better co-deliver systems.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Portadores de Fármacos
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Produtos do Gene tat
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Núcleo Celular
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Técnicas de Transferência de Genes
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Quitosana
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Vetores Genéticos
Limite:
Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2018
Tipo de documento:
Article