Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality.
Cryobiology
; 80: 26-29, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-29287896
ABSTRACT
Human spermatozoa cryopreservation is an important means of assisted reproductive technology and male fertility preservation. Although this technique is particularly useful, sperm cryopreservation significantly reduces the quality of spermatozoa after freezing and thawing. The objective of the study is to examine the efficacy of mitochondria-targeted antioxidant MitoTEMPO in improving sperm quality during semen cryopreservation processes. Semen samples were collected and cryopreserved in extenders containing different concentrations (0.0, 0.5, 5, 50, and 500⯵M) of MitoTEMPO. Sperm motility, viability, membrane integrity, mitochondrial membrane potential and antioxidant activities were measured and analyzed. The results showed that the addition of MitoTEMPO (5-50⯵M) significantly improved post-thaw sperm motility, viability, membrane integrity and mitochondrial membrane potential (Pâ¯<â¯.05). Meanwhile, antioxidant enzymes activities were enhanced and MDA content were decreased in the group supplemented with MitoTEMPO. In conclusion, mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality and antioxidant enzymes profile.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Organofosforados
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Piperidinas
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Preservação do Sêmen
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Criopreservação
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Crioprotetores
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Antioxidantes
Limite:
Humans
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Male
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China