Effect of idebenone on bone marrow mesenchymal stem cells in vitro.
Mol Med Rep
; 17(4): 5376-5383, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29393352
ABSTRACT
In recent years, stem cell research has continued to benefit from its crossover with chemistry, particularly the investigation of small molecular drugs modulating specific targets to regulate stem cell fate. Idebenone (IDB) is a yellow crystalline powder that is used in the treatment of chronic cerebrovascular diseases. The objective of the present study was to examine whether IDB had an influence on bone marrowderived mesenchymal stem cells (BMSCs) extracted from the bone marrow of SpragueDawley rats. The effects of IDB on cell proliferation, cell cloning and migration were investigated. Cell cycle, apoptosis, DAPI nuclear staining and senescenceassociated ßgalactosidase (SAßgal) staining were also examined. The results revealed that IDB at suitable concentrations enhanced cell cloning capacity, promoted the proliferation of BMSCs, delayed cellular senescence, and inhibited cell apoptosis and migration. Western blot analysis indicated that IDB increased the expression of Bcell lymphoma 2 (Bcl2), signal transducer and activator of transcription3, Nanog, octamerbinding transcription factor 4, Ecadherin, proliferating cell nuclear antigen, cyclinD1 and cyclinD3, and decreased the expression of Bcl2associated X protein, cleaved caspase3, Ncadherin, vimentin and αsmooth muscle actin. In conclusion, these experiments confirmed that IDB in low doses had no toxic effect and may exert protective effects on BMSCs.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ubiquinona
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article