Colorimetric aptasensors for determination of tobramycin in milk and chicken eggs based on DNA and gold nanoparticles.
Food Chem
; 249: 98-103, 2018 May 30.
Article
em En
| MEDLINE
| ID: mdl-29407938
ABSTRACT
Colorimetric aptasensors were designed for detection of tobramycin (TOB) based on unmodified gold nanoparticles (AuNPs) and single-strand DNA (ssDNA). In the absence of TOB, the DNA aptamer was coated on the surface of AuNPs to keep it against salt-induced aggregation. In the presence of TOB, aptamer will bind with TOB and detach from the surface of AuNPs because of higher affinities between aptamer and TOB. Then less protection of DNA may result in the aggregation of AuNPs by salt and an apparent color change from red to purple-blue. The developed aptasensors showed a high selectivity and sensitivity for TOB detection. The linearity range and the detection limit were 40-200â¯nM and 23.3â¯nM respectively. The validity of the procedure and applicability of aptasensors were successfully used to detect TOB in milk and chicken eggs, and the results were excellent in accord with the values obtained by spectrofluorimetric detection.
Palavras-chave
Aptasensor; Chloroauric acid (PubChem CID: 28133); Colorimetric; Food products; Kanamycin (PubChem CID: 6032); Streptomycin (PubChem CID: 19649); Sulfadimethoxine (PubChem CID: 5323); Sulfamethoxazole (PubChem CID: 5329); Tobramycin; Tobramycin (PubChem CID: 36294); Trichloroacetic acid (PubChem CID: 6421); UVVis spectrophotometer; l-Alanine (PubChem CID: 5950); l-Arginine (PubChem CID: 6322); l-Lysine (PubChem CID: 5962); l-Proline (PubChem CID: 145742); l-Tryptophan (PubChem CID: 6305); l-Tyrosine (PubChem CID: 6057)
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tobramicina
/
DNA
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Colorimetria
/
Leite
/
Ovos
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Aptâmeros de Nucleotídeos
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Ouro
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Antibacterianos
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
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UNITED KINGDOM