Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry.
Biol Cell
; 57(1): 1-7, 1986.
Article
em En
| MEDLINE
| ID: mdl-2945606
ABSTRACT
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of the whole cell and its organelles. Rhodamine 123 is a vital fluorescent dye used in flow cytometry. As it is specifically concentrated in mitochondria because of the transmembrane potential that these organelles maintain in living cells, rhodamine 123 is thus a useful probe for monitoring the abundance and activity of mitochondria. A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rodaminas
/
Xantenos
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
Biol Cell
Ano de publicação:
1986
Tipo de documento:
Article