Reconstituting the 4-Strand DNA Strand Exchange.
Methods Enzymol
; 600: 285-305, 2018.
Article
em En
| MEDLINE
| ID: mdl-29458763
ABSTRACT
Proteins of the Rad51 family play a key role in homologous recombination by carrying out DNA strand exchange. Here, we present the methodology and the protocols for the 4-strand exchange between gapped circular DNA and homologous linear duplex DNA promoted by human Rad51 and Escherichia coli RecA orthologs. This reaction includes formation of joint molecules and their extension by branch migration in a polar manner. The presented methodology may be used for reconstitution of the medial-to-late stages of homologous recombination in vitro as well as for investigation of the mechanisms of branch migration by helicase-like proteins, e.g., Rad54, BLM, or RecQ1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinases Rec A
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DNA Circular
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Proteínas de Escherichia coli
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Rad51 Recombinase
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Ácidos Nucleicos Heteroduplexes
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos