[Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical trap].
Prikl Biokhim Mikrobiol
; 53(2): 234-43, 2017.
Article
em Ru
| MEDLINE
| ID: mdl-29509378
A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. pseudotuberculosis in colonization of a macroorganism. The developed methodical approaches can be applied to the study of molecular mechanisms of the pathogenesis of pseudotuberculosis and other infectious diseases to improve antiepidemic service.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Yersinia pseudotuberculosis
/
Lipopolissacarídeos
/
Imunoconjugados
/
Macrófagos
/
Anticorpos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ru
Revista:
Prikl Biokhim Mikrobiol
Ano de publicação:
2017
Tipo de documento:
Article
País de publicação:
Federação Russa