Antibody-Conjugated Silica-Modified Gold Nanorods for the Diagnosis and Photo-Thermal Therapy of Cryptococcus neoformans: an Experiment In Vitro.
Nanoscale Res Lett
; 13(1): 77, 2018 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-29516271
ABSTRACT
BACKGROUND:
Cryptococcus neoformans is an encapsulated yeast. There is still little quick and effective solution for the diagnosis or treatment of C. neoformans infection at an early stage in clinical. Antibody-conjugated silica-modified gold nanorods (GNR-SiO2-Ab) can conjugate C. neoformans selectively. It may provide a possibility for treatment of cryptococcosis safely and effectively.METHODS:
Gold nanorods (GNRs) were synthesized according to the seed-mediated template-assisted protocol. Anti-C. neoformans antibody was covalently anchored on the surface of GNRs with silane coupling agent. In vitro computer tomography imaging was performed to explore the diagnostic effect of the GNR-SiO2-Ab. The viability of cells was evaluated to confirm the photo-thermal therapy effect of GNR-SiO2-Ab combined with near-infrared (NIR) laser light.RESULTS:
GNR-SiO2-Ab has a potential application as a positive X-ray/CT imaging contrast agent. An antibody can induce a much greater aggregation of GNRs by binding to the surface of C. neoformans cells resulting in a much higher attenuation values than ever. After irradiation, C. neoformans cells suffered photo-thermal damages and the normal structure of cells were destroyed. The viability of cells reduced significantly compared to the untreated cells.CONCLUSIONS:
Our work confirmed that antibody-conjugated silica-modified gold nanorods could enhance X-ray attenuation of C. neoformans cells in CT images. And immune GNRs, which were mediated by antibodies, could increase the effects of NIR-induced photo-thermal therapy in C. neoformans cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
/
Guideline
Idioma:
En
Revista:
Nanoscale Res Lett
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA