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The biological effects of long-term exposure of human bronchial epithelial cells to total particulate matter from a candidate modified-risk tobacco product.
van der Toorn, Marco; Sewer, Alain; Marescotti, Diego; Johne, Stephanie; Baumer, Karin; Bornand, David; Dulize, Remi; Merg, Celine; Corciulo, Maica; Scotti, Elena; Pak, Claudius; Leroy, Patrice; Guedj, Emmanuel; Ivanov, Nikolai; Martin, Florian; Peitsch, Manuel; Hoeng, Julia; Luettich, Karsta.
Afiliação
  • van der Toorn M; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland. Electronic address: Marco.vanderToorn@pmi.com.
  • Sewer A; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Marescotti D; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Johne S; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Baumer K; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Bornand D; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Dulize R; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Merg C; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Corciulo M; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Scotti E; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Pak C; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Leroy P; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Guedj E; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Ivanov N; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Martin F; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Peitsch M; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Hoeng J; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
  • Luettich K; PMI R&D, Philip Morris Products S.A. (Part of Philip Morris International group of companies), Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Toxicol In Vitro ; 50: 95-108, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29524472
Cigarette smoking is the leading cause of preventable lung cancer (LC). Reduction of harmful constituents by heating rather than combusting tobacco may have the potential to reduce the risk of LC. We evaluated functional and molecular changes in human bronchial epithelial BEAS-2B cells following a 12-week exposure to total particulate matter (TPM) from the aerosol of a candidate modified-risk tobacco product (cMRTP) in comparison with those following exposure to TPM from the 3R4F reference cigarette. Endpoints linked to lung carcinogenesis were assessed. Four-week 3R4F TPM exposure resulted in crisis and epithelial to mesenchymal transition (EMT) accompanied by decreased barrier function and disrupted cell-to-cell contacts. By week eight, cells regained E-cadherin expression, suggesting that EMT was reversible. Increased levels of inflammatory mediators were noted in cells treated to 3R4F TPM but not in cells treated to the same or a five-fold higher concentration of cMRTP TPM. A 20-fold higher concentration of cMRTP TPM increased oxidative stress and DNA damage and caused reversible EMT. Anchorage-independent growth was observed in cells treated to 3R4F or a high concentration of cMRTP TPM. 3R4F TPM-derived clones were invasive, while cMRTP TPM-derived clones were not. Long-term exposure to TPM from the cMRTP had a lower biological impact on BEAS-2B cells compared with that of exposure to TPM from 3R4F.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Epiteliais / Material Particulado / Produtos do Tabaco Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Epiteliais / Material Particulado / Produtos do Tabaco Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de publicação: Reino Unido