Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector.
Virol J
; 15(1): 49, 2018 03 20.
Article
em En
| MEDLINE
| ID: mdl-29558962
ABSTRACT
BACKGROUND:
DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide), no similar study has been conducted using T7 phage.METHODS:
In this study, we engineeredT7 phage as a DNA targeting delivery vector to facilitate cellular internalization. We constructed recombinant T7 phages that displayed Tat peptide on their surface and carried eukaryotic expression box (EEB) as a part of their genomes (T7-EEB-Tat).RESULTS:
We demonstrated that T7 phage harboring foreign gene insertion had packaged into infective progeny phage particles. Moreover, when mammalian cells that were briefly exposed to T7-EEB-Tat, expressed a significant higher level of the marker gene with the control cells infected with the wide type phage without displaying Tat peptides.CONCLUSION:
These data suggested that the potential of T7 phage as an effective delivery vector for DNA vaccine transfer.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriófago T7
/
Vacinas de DNA
/
Vetores Genéticos
Limite:
Humans
Idioma:
En
Revista:
Virol J
Assunto da revista:
VIROLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China