BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.
Genome Med
; 10(1): 20, 2018 03 20.
Article
em En
| MEDLINE
| ID: mdl-29558968
ABSTRACT
B cells play a critical role in the immune response by producing antibodies, which display remarkable diversity. Here we describe a bioinformatic pipeline, BALDR (BCR Assignment of Lineage using De novo Reconstruction) that accurately reconstructs the paired heavy and light chain immunoglobulin gene sequences from Illumina single-cell RNA-seq data. BALDR was accurate for clonotype identification in human and rhesus macaque influenza vaccine and simian immunodeficiency virus vaccine induced vaccine-induced plasmablasts and naïve and antigen-specific memory B cells. BALDR enables matching of clonotype identity with single-cell transcriptional information in B cell lineages and will have broad application in the fields of vaccines, human immunodeficiency virus broadly neutralizing antibody development, and cancer.BALDR is available at https//github.com/BosingerLab/BALDR .
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Cadeias Pesadas de Imunoglobulinas
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Cadeias Leves de Imunoglobulina
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Análise de Sequência de RNA
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Biologia Computacional
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Análise de Célula Única
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Genome Med
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos