Cell type transcriptome atlas for the planarian Schmidtea mediterranea.
Science
; 360(6391)2018 05 25.
Article
em En
| MEDLINE
| ID: mdl-29674431
ABSTRACT
The transcriptome of a cell dictates its unique cell type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. We generated data for 66,783 cells, defining transcriptomes for known and many previously unknown planarian cell types and for putative transition states between stem and differentiated cells. We also uncovered regionally expressed genes in muscle, which harbors positional information. Identifying the transcriptomes for potentially all cell types for many organisms should be readily attainable and represents a powerful approach to metazoan biology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Planárias
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Atlas como Assunto
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Células
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Análise de Sequência de RNA
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Perfilação da Expressão Gênica
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Análise de Célula Única
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Transcriptoma
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos