Monoclonal antibody internalization and degradation during modulation of the CD3/T-cell receptor complex.
Cell Immunol
; 116(1): 52-9, 1988 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-2971460
ABSTRACT
Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to investigate their fate in Jurkat T cells. We demonstrated that all mAb were progressively internalized and degraded in Jurkat T cells and that this degradation was inhibited by chloroquine, an inhibitor of lysosomal degradation enzymes. The sequestration of anti-CD3 mAb in acid compartments was furthermore shown using cytofluorometry. All together our results show that antibodies against CD3 or against TCR follow the same endocytic pathway.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Antígenos de Diferenciação de Linfócitos T
Limite:
Humans
Idioma:
En
Revista:
Cell Immunol
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
França