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Molecular and biological characterization of the human-bovine rotavirus-based reassortant rotavirus.
Zafari, Ehsan; Soleimanjahi, Hoorieh; Mohammadi, Ashraf; Teimoori, Ali; Mahravani, Homayon.
Afiliação
  • Zafari E; Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Soleimanjahi H; Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: soleim_h@modares.ac.ir.
  • Mohammadi A; Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
  • Teimoori A; Department of Virology, Ahwaz Joundishpor University of Medical Sciences, Ahvaz, IR Iran.
  • Mahravani H; Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
Microb Pathog ; 121: 65-69, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29753872
Rotaviruses (RV) are the leading cause of acute infantile gastroenteritis, associated with elevated mortality in low-income countries. Morbidity and mortality, length and rates of hospitalization due to RV gastroenteritis are dropping. Improving the quality of newborns life is an ongoing challenge for health-care providers. In this study, homemade reassortant human-bovine rotavirus was developed and biological activity and molecular characterization of candidate vaccine were evaluated for the vaccine stability. Virus titration and purification of reassortant rotavirus strains were evaluated by plaque assays, electropherotyping. The genetic stability after first, third and sixth passage was by sequencing. Due to WHO recommendation, developingment of national capacity for vaccine production in appropriate quantities and at affordable prices is the cornerstone of developing global vaccination policies. Such studies are critical to producing national vaccines and modeling herd protection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Vírus Reordenados / Rotavirus / Gastroenterite / Genes Virais Limite: Animals / Humans País/Região como assunto: Asia Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Irã País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Vírus Reordenados / Rotavirus / Gastroenterite / Genes Virais Limite: Animals / Humans País/Região como assunto: Asia Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Irã País de publicação: Reino Unido