Molecular cloning, characterization and expression analysis of spexin in spotted scat (Scatophagus argus).
Gen Comp Endocrinol
; 266: 60-66, 2018 09 15.
Article
em En
| MEDLINE
| ID: mdl-29753927
ABSTRACT
Spexin (Spx), a novel neuropeptide, composed of 14 amino acid residues, is evolutionally conserved from fish to mammals. It has been suggested that Spx has pleiotropic functions in mammals. However, reports about Spx are very limited. To clarify the roles of Spx in the regulation of reproduction and food-intake in the spotted scat, the spx (ssspx) gene was cloned and analyzed. Analysis of the tissue distribution by RT-PCR showed that ssspx expression was widespread. During ovary development, expression of ssspx was found to be highest in phase II, moderate in phase III, and at its lowest level in phase IV. Ssspx expression was significantly down-regulated in the hypothalamus after treatment with E2 both in vitro and in vivo. A significant increase of ssspx was observed after 2 and 7â¯days of food deprivation. However, the ssspx transcript levels in the 7â¯day fasting group decreased significantly after refeeding 3â¯h after the scheduled feeding time. This suggests that ssSpx may be involved in the regulation of reproduction and food-intake in the spotted scat.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perciformes
/
Perfilação da Expressão Gênica
/
Hormônios Peptídicos
Limite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China