A hyaluronan-based nanosystem enables combined anti-inflammation of mTOR gene silencing and pharmacotherapy.
Carbohydr Polym
; 195: 339-348, 2018 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-29804985
ABSTRACT
Accompanied by overproduction of oxidants and reduction of pH, inflammation is closely related to many diseases such as cancer, atherosclerosis, and asthma. Besides chemotherapeutic agents, the potential regulative role of autophagy in inflammation is being actively investigated. RNA interference (RNAi)-based gene therapy is widely explored for clinical therapy but seriously restricted by lack of suitable carriers. In this study, we synthesized a hyaluronan-based ROS-sensitive polymer which was expected to release loaded chemical drugs in inflammatory environment and further developed a stable and nontoxic co-delivery nanosystem of siRNA targeting autophagy suppressive gene and chemotherapeutic agents. The in vitro transfection study of this nanosystem revealed improved intracellular accumulation of siRNA and excellent gene silencing efficacy comparable to that of conventional cationic liposome. Moreover, the mRNA expression of inflammatory cytokines was remarkably decreased by our nanosystem. Considering its biocompatibility, transfection efficacy, and anti-inflammatory capability, this co-delivery nanosystem proclaimed to be a promising combined therapeutic strategy for enhanced anti-inflammatory therapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
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Inativação Gênica
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Nanopartículas
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Serina-Treonina Quinases TOR
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Ácido Hialurônico
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China