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16S metagenomic analysis reveals adaptability of a mixed-PAH-degrading consortium isolated from crude oil-contaminated seawater to changing environmental conditions.
Muangchinda, Chanokporn; Rungsihiranrut, Adisan; Prombutara, Pinidphon; Soonglerdsongpha, Suwat; Pinyakong, Onruthai.
Afiliação
  • Muangchinda C; Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Thailand.
  • Rungsihiranrut A; Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Thailand.
  • Prombutara P; Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Thailand.
  • Soonglerdsongpha S; Environmental Technology Research Department, PTT Research and Technology Institute, PTT Public Company Limited, Ayutthaya, Thailand.
  • Pinyakong O; Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Thailand; Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Thailand; Research Program on Remediation Technologies for Pe
J Hazard Mater ; 357: 119-127, 2018 09 05.
Article em En | MEDLINE | ID: mdl-29870896
ABSTRACT
A bacterial consortium, named SWO, was enriched from crude oil-contaminated seawater from Phrao Bay in Rayong Province, Thailand, after a large oil spill in 2013. The bacterial consortium degraded a polycyclic aromatic hydrocarbon (PAH) mixture consisting of phenanthrene, anthracene, fluoranthene, and pyrene (50 mg L-1 each) by approximately 73%, 69%, 52%, and 48%, respectively, within 21 days. This consortium exhibited excellent adaptation to a wide range of environmental conditions. It could degrade a mixture of four PAHs under a range of pH values (4.0-9.0), temperatures (25 °C-37 °C), and salinities (0-10 g L-1 with NaCl). In addition, this consortium degraded 20-30% of benzo[a]pyrene and perylene (10 mg L-1 each), high molecular weight PAHs, in the presence of other PAHs within 35 days, and degraded 40% of 2% (v/v) crude oil within 20 days. The 16S rRNA gene amplicon sequencing analysis demonstrated that Pseudomonas and Methylophaga were the dominant genera of consortium SWO in almost all treatments, while Pseudidiomarina, Thalassospira and Alcanivorax were predominant under higher salt concentrations. Moreover, Pseudomonas and Alcanivorax were dominant in the crude oil-degradation treatment. Our results suggest that the consortium SWO maintained its biodegradation ability by altering the bacterial community profile upon encountering changes in the environmental conditions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Água do Mar / Poluentes Químicos da Água / Petróleo Idioma: En Revista: J Hazard Mater Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Tailândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrocarbonetos Policíclicos Aromáticos / Água do Mar / Poluentes Químicos da Água / Petróleo Idioma: En Revista: J Hazard Mater Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Tailândia
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