Investigation of BK Virus by Real-Time Polymerase Chain Reaction in Patients With Allogeneic Bone Marrow Transplantation.
Transplant Proc
; 50(5): 1510-1513, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29880379
ABSTRACT
INTRODUCTION:
BK virus (BKV) is a common human polyomavirus and causes latent infection. Especially in immunosuppressive patients, early diagnosis and treatment are very important in reducing the high mortality rate. In this study, we investigated BKV DNA in serum and plasma and urine specimens by real-time polymerase chain reaction (PCR) method in allogeneic hematopoietic stem cell transplantation patients. MATERIALS ANDMETHODS:
BKV DNA was isolated in QIAsymphony SP/AS (Hilden, Germany) equipment using QIAsymphony DSP Virus/Pathogen Midi Kit, Version 1 (QIAGEN, Hilden, Germany) in urine and serum samples collected from 100 patients post-transplantation. Artus BKV QS-RGQ, V1 (Qiagen, Hilden, Germany) kit for BKV PCR was prepared according to the company recommendations and loaded on Qiagen Rotor Gene (Hilden, Germany). It was evaluated with PCR, and >27 copies/mL was considered as positive.RESULTS:
BKV DNA was positive in 57% of the urine specimens obtained; only 25% of the patients were found to have a significant BKV burden in the urine according to the American Society of Transplantation, suggesting a risk of developing nephropathy. Serum samples of the same patients were negative for BKV DNA in 94 cases and they were positive for BKV DNA at interval between 44 and 319 copies/mL in 6 patients, and none of the patients had clinically significant BKV DNA in serum samples.CONCLUSIONS:
Monitoring the viral load with urine samples were thought to be more convenient for the detection of BKV reactivation in our study.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Vírus BK
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Transplante de Células-Tronco Hematopoéticas
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Infecções por Polyomavirus
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Screening_studies
Limite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
Europa
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2018
Tipo de documento:
Article