[Chiral separation of fourteen amino alcohols by nonaqueous capillary electrophoresis].
Yao Xue Xue Bao
; 51(8): 1297-301, 2016 08.
Article
em Zh
| MEDLINE
| ID: mdl-29906027
ABSTRACT
We developed a new method for chiral separation of fourteen amino alcohols by nonaqueous capillary electrophoresis (NACE) with the D-(+)-gluconic acid δ-lactone-boric acid complex as chiral selector. In order to achieve good enantioseparation, the effects of D-(+)-gluconic acid δ-lactone and boric acid concentrations, triethylamine concentration, as well as capillary temperature were systematically investigated. The optimized conditions were identified as follows an uncoated fused silica capillary of 50 µm ID with a total length (L(tot)) of 55 cm and an effective length (L(eff)) of 45 cm; 200 mmol·L(-1) D-(+)-gluconic acid δ-lactone, 80 mmol·L(-1) boric acid, and 57.4 mmol·L(-1) triethylamine in methanol; positive pressure injection at 2.9 psi for 2 s; capillary temperature, 25 ± 0.2 â; applied voltage, +15 k V; detection wavelength, 214 nm. Under the optimized conditions, a good chiral resolution was achieved in most of the tested drugs. This method provides a foundation for the development and application of new chiral selectors of polyhydroxy compound-boric acid complexes in chiral drugs analysis by NACE.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Amino Álcoois
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Ano de publicação:
2016
Tipo de documento:
Article