Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm.
J Vis Exp
; (136)2018 06 07.
Article
em En
| MEDLINE
| ID: mdl-29939175
ABSTRACT
The present protocol describes the Conditioned Place Preference (CPP) as a model of relapse in drug addiction. In this model, animals are first trained to acquire a conditioned place preference in a drug-paired compartment, and after the post-conditioning test, they perform several sessions to extinguish the established preference. The CPP permits the evaluation of the conditioned rewarding effects of drugs related to environmental cues. Then, the extinguished CPP can be robustly reinstated by the non-contingent administration of a priming dose of the drug, and by exposure to stressful stimuli. Both methods will be explained here. When the animal reinitiates the behavioral response, a reinstatement of the conditioned reward is considered to have taken place. The main advantages of this protocol are that it is non-invasive, inexpensive, and simple with good validity criteria. In addition, it allows the study of different environmental manipulations, such as stress or diet, which can modulate relapse into drug seeking behaviors. However, one limitation is that if the researcher aims to explore the motivation and primary reinforcing effects of the drug, it should be complemented with self-administration procedures, as they involve operant responses of animals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Terapia Comportamental
/
Condicionamento Operante
/
Comportamento de Procura de Droga
Tipo de estudo:
Prognostic_studies
Aspecto:
Patient_preference
Limite:
Animals
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2018
Tipo de documento:
Article