Effect of phlorotannins on myofibroblast differentiation and ECM protein expression in transforming growth factor ß1induced nasal polypderived fibroblasts.
Int J Mol Med
; 42(4): 2213-2220, 2018 Oct.
Article
em En
| MEDLINE
| ID: mdl-30015862
ABSTRACT
Phlorotannins (PTNs), a group of phenolic compounds from seaweeds, have diverse bioactivities. However, there has been no report on their antifibrotic effects during nasal polyp (NP) formation. In the present study, the effect of PTNs on transforming growth factor (TGF)ß1induced profibrotic responses in nasal polypderived fibroblasts (NPDFs) were determined and the relevant signaling pathways were investigated. The expression levels of collagen type1 (Col1) and fibronectin in NP tissues were measured by western blot analysis and immunohistochemistry. The NPDFs were treated with TGFß1 (1 ng/ml) in the presence or absence of PTNs (530 µg/ml). The expression levels of αsmooth muscle actin (αSMA), Col1, fibronectin, and phosphorylatedsmall mothers against decapentaplegic (Smad)2/3 in NPDFs were measured by western blot analysis. The contractile activity of the NPDFs was determined by a collagen gel contraction assay. Col1 and fibronectin proteins were found to be expressed in NP tissues. PTNs had no significant cytotoxic effect on TGFß1induced NPDFs. TGFß1 induced the expression αSMA, Col1 and fibronectin, and stimulated fibroblastmediated contraction of collagen gel. However, pretreatment with PTNs inhibited the expression of these proteins. The inhibitory effects were mediated through the suppression of Smad2/3 signaling pathways in TGFß1induced NPDFs. These resulted suggested that PTNs may be important in inhibiting myofibroblast differentiation and extracellular matrix protein accumulation in NP formation through the Smad2/3 signaling pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Taninos
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Diferenciação Celular
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Regulação da Expressão Gênica
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Pólipos Nasais
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Proteínas da Matriz Extracelular
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Fator de Crescimento Transformador beta1
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Miofibroblastos
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article