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Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene.
Shang, Junjun; Li, Yan; Yang, Ruiheng; Wang, Ying; Mao, Wenjun; Tang, Lihua; Wu, Yingying; Nakazawa, Takehito; Honda, Yoichi; Li, Yan; Bao, Dapeng.
Afiliação
  • Shang J; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Li Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Yang R; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Wang Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Mao W; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Tang L; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Wu Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China.
  • Nakazawa T; Laboratory of Forest Biochemistry, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake-cho, Sakyoku-ku, Kyoto 606-8502, Japan.
  • Honda Y; Laboratory of Forest Biochemistry, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake-cho, Sakyoku-ku, Kyoto 606-8502, Japan.
  • Li Y; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China. Electronic address: swallow2005@live.cn.
  • Bao D; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, PR China. Electronic address: baodp@hotmail.com.
J Microbiol Methods ; 152: 7-9, 2018 09.
Article em En | MEDLINE | ID: mdl-30017848
ABSTRACT
We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transformação Genética / Proteínas Fúngicas / Biomarcadores / Marcadores Genéticos / Pleurotus Idioma: En Revista: J Microbiol Methods Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transformação Genética / Proteínas Fúngicas / Biomarcadores / Marcadores Genéticos / Pleurotus Idioma: En Revista: J Microbiol Methods Ano de publicação: 2018 Tipo de documento: Article