Enhanced glycolysis in the process of renal fibrosis aggravated the development of chronic kidney disease.
Eur Rev Med Pharmacol Sci
; 22(13): 4243-4251, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-30024614
ABSTRACT
OBJECTIVE:
To explore the effect of glycometabolism on renal fibrosis and its underlying mechanism. MATERIALS ANDMETHODS:
For in vivo experiments, unilateral ureteral obstruction (UUO) mouse model was constructed to induce renal interstitial fibrosis. Fibrosis and proliferation indicators in renal tissues were detected to observe the fibroblast phenotype changes during the process of renal fibrosis. Moreover, mRNA and protein levels of key enzymes in glycometabolism were also detected. For in vitro experiments, plasmid transfection was performed to overexpress pyruvate kinase M2 (PKM2) to explore the relationship between PKM2 and renal interstitial fibrosis. Energy metabolism monitoring was performed to detect changes in aerobic glycolysis and oxidative phosphorylation during the process of TGF-ß1-induced fibroblast phenotype changes.RESULTS:
Fibroblast phenotype was changed. Both fibrosis and proliferation indicators were upregulated during renal fibrosis. Meanwhile, elevated expressions of key enzymes in glycometabolism and metabolic reprogramming of fibroblasts were observed. Overexpressed PKM2 activated fibroblasts and induced renal interstitial fibrosis, accompanied by increased glycometabolism level.CONCLUSIONS:
Metabolic reprogramming promoted renal interstitial fibrosis, which leads to alteration of cell energy metabolism model.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Insuficiência Renal Crônica
/
Fibroblastos
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Glicólise
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Rim
Limite:
Animals
/
Humans
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Male
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China