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Nanoscale Mapping Functional Sites on Nanoparticles by Points Accumulation for Imaging in Nanoscale Topography (PAINT).
Delcanale, Pietro; Miret-Ontiveros, Bernat; Arista-Romero, Maria; Pujals, Silvia; Albertazzi, Lorenzo.
Afiliação
  • Delcanale P; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology , Baldiri Reixac 15-21 , 08028 Barcelona , Spain.
  • Miret-Ontiveros B; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology , Baldiri Reixac 15-21 , 08028 Barcelona , Spain.
  • Arista-Romero M; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology , Baldiri Reixac 15-21 , 08028 Barcelona , Spain.
  • Pujals S; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology , Baldiri Reixac 15-21 , 08028 Barcelona , Spain.
  • Albertazzi L; Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology , Baldiri Reixac 15-21 , 08028 Barcelona , Spain.
ACS Nano ; 12(8): 7629-7637, 2018 08 28.
Article em En | MEDLINE | ID: mdl-30048592
The ability of nanoparticles to selectively recognize a molecular target constitutes the key toward nanomedicine applications such as drug delivery and diagnostics. The activity of such devices is mediated by the presence of multiple copies of functional molecules on the nanostructure surface. Therefore, understanding the number and the distribution of nanoparticle functional groups is of utmost importance for the rational design of effective materials. Analytical methods are available, but to obtain quantitative information at the level of single particles and single functional sites, i. e., going beyond the ensemble, remains highly challenging. Here we introduce the use of an optical nanoscopy technique, DNA points accumulation for imaging in nanoscale topography (DNA-PAINT), to address this issue. Combining subdiffraction spatial resolution with molecular selectivity and sensitivity, DNA-PAINT provides both geometrical and functional information at the level of a single nanostructure. We show how DNA-PAINT can be used to image and quantify relevant functional proteins such as antibodies and streptavidin on nanoparticles and microparticles with nanometric accuracy in 3D and multiple colors. The generality and the applicability of our method without the need for fluorescent labeling hold great promise for the robust quantitative nanocharacterization of functional nanomaterials.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Nanotecnologia / Nanoestruturas / Imagem Óptica Idioma: En Revista: ACS Nano Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Espanha País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Nanotecnologia / Nanoestruturas / Imagem Óptica Idioma: En Revista: ACS Nano Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Espanha País de publicação: Estados Unidos