Your browser doesn't support javascript.
loading
A comparative ultrastructure study of storage cells in the eutardigrade Richtersius coronifer in the hydrated state and after desiccation and heating stress.
Czerneková, Michaela; Janelt, Kamil; Student, Sebastian; Jönsson, K Ingemar; Poprawa, Izabela.
Afiliação
  • Czerneková M; Department of Environmental Science and Bioscience, Kristianstad University, Kristianstad, Sweden.
  • Janelt K; Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
  • Student S; Faculty of Medicine, Charles University, Prague, Czech Republic.
  • Jönsson KI; Department of Animal Histology and Embryology, University of Silesia in Katowice, Katowice, Poland.
  • Poprawa I; Silesian University of Technology, Institute of Automatic Control, Gliwice, Poland.
PLoS One ; 13(8): e0201430, 2018.
Article em En | MEDLINE | ID: mdl-30096140
Tardigrades represent an invertebrate phylum with no circulatory or respiratory system. Their body cavity is filled with free storage cells of the coelomocyte-type, which are responsible for important physiological functions. We report a study comparing the ultrastructure of storage cells in anhydrobiotic and hydrated specimens of the eutardigrade Richtersius coronifer. We also analysed the effect of temperature stress on storage cell structure. Firstly, we verified two types of ultrastructurally different storage cells, which differ in cellular organelle complexity, amount and content of reserve material and connection to oogenetic stage. Type I cells were found to differ ultrastructurally depending on the oogenetic stage of the animal. The main function of these cells is energy storage. Storage cells of Type I were also observed in the single male that was found among the analysed specimens. The second cell type, Type II, found only in females, represents young undifferentiated cells, possibly stem cells. The two types of cells also differ with respect to the presence of nucleolar vacuoles, which are related to oogenetic stages and to changes in nucleolic activity during oogenesis. Secondly, this study revealed that storage cells are not ultrastructurally affected by six months of desiccation or by heating following this desiccation period. However, heating of the desiccated animals (tuns) tended to reduce animal survival, indicating that long-term desiccation makes these animals more vulnerable to heat stress. We confirmed the degradative pathways during the rehydration process after desiccation and heat stress. Our study is the first to document two ultrastructurally different types of storage cells in tardigrades and reveals new perspectives for further studies of tardigrade storage cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resposta ao Choque Térmico / Estruturas Animais / Dessecação / Invertebrados Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Suécia País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resposta ao Choque Térmico / Estruturas Animais / Dessecação / Invertebrados Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Suécia País de publicação: Estados Unidos