Scanning spectrophotometry for the dynamic study of tissue respiration in intact organs.
J Biomed Eng
; 8(2): 166-70, 1986 Apr.
Article
em En
| MEDLINE
| ID: mdl-3012201
ABSTRACT
For use with intact perfused organs a spectrophotometer system has been developed, both for dual-wavelength absorption measurements and for spectral scanning. A monochromator is used for illumination and scanning in the visible and near infrared. Optic fibres conduct light to the specimen under examination and from the specimen to a detecting photomultiplier. System control is exercised by a microcomputer, which also processes the collected data. The performance of the system on isolated perfused rat heart is demonstrated, in the spectral scanning mode and in the dual-wavelength mode, by studying simultaneously the kinetics of cytochrome aa3, and myoglobin oxidation-reduction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
/
Espectrofotometria
/
Miocárdio
Limite:
Animals
Idioma:
En
Revista:
J Biomed Eng
Ano de publicação:
1986
Tipo de documento:
Article