Propagation and Purification of Ectromelia Virus.
Curr Protoc Microbiol
; 51(1): e65, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30281950
Ectromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Mousepox makes an excellent small animal model for smallpox to investigate pathogenesis, vaccine and antiviral agent testing, host-virus interactions, and immune and inflammatory responses. The availability of a wide variety of inbred, congenic, and gene-knockout mice allows detailed analyses of the host response. ECTV mutant viruses lacking one or more genes encoding immunomodulatory proteins are being used in numerous studies in conjunction with wild-type or gene-knockout mice to study the functions of these genes in host-virus interactions. The methods used for propagation of ECTV in cell culture, purification, and quantification of infectious particles through viral plaque assay are described. © 2018 by John Wiley & Sons, Inc.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Placa Viral
/
Cultura de Vírus
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Carga Viral
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Vírus da Ectromelia
Limite:
Animals
Idioma:
En
Revista:
Curr Protoc Microbiol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Austrália
País de publicação:
Estados Unidos