Human amniotic membrane as differentiating matrix for in vitro chondrogenesis.
Regen Med
; 13(7): 821-832, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30299207
ABSTRACT
AIM:
The aim of the present study is to use human amniotic membrane (HAM) for in vitro chondrogenesis of placenta-derived mesenchymal stem cells (MSCs) and umbilical cord-derived MSCs. MATERIALS &METHODS:
MSCs from the placenta and umbilical cord were isolated, characterized by immunophenotyping and after analyzing their rate of proliferation, cytotoxicity and viability, chondrogenesis was performed on plastic adherent surface and on HAM.RESULTS:
Successfully isolated and characterized placenta-derived MSCs and umbilical cord-derived MSCs revealed positive expression of MSCs markers CD90, CD73, CD105 and CD49d, while they were negative for CD45. Both types of cells in the presence of chondrogenic induction medium on plastic adherent surface and HAM showed aggregates of proteoglycan and strong expression of COL2A1 (collagen 2) and ACAN1 (aggrecan).CONCLUSION:
HAM supported proliferation as well as chondrogenesis of MSCs and provide novelty of HAM utilization as an efficient natural delivery matrix for stem cell transplantation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
/
Condrogênese
/
Células-Tronco Mesenquimais
/
Âmnio
Limite:
Humans
Idioma:
En
Revista:
Regen Med
Ano de publicação:
2018
Tipo de documento:
Article