Improved oxygen storage capacity of haemoglobin submicron particles by one-pot formulation.
Artif Cells Nanomed Biotechnol
; 46(sup3): S964-S972, 2018.
Article
em En
| MEDLINE
| ID: mdl-30388380
ABSTRACT
The coprecipitation-cross-linking-dissolution (CCD) technique for protein submicron particle fabrication was improved by omitting one preparation step using the macromolecular cross-linker, periodate-oxidized dextran (Odex, M.W. of 40 and 70 kDa). The coprecipitation and cross-linking of haemoglobin (Hb) were combined in one single step since the cross-linker is incorporated into the inorganic template, MnCO3, together with the protein. After removal of the MnCO3 templates by EDTA, the amount of entrapped Hb was 60 to 70% of the initial amount. This technique provides deformable Hb submicron particles (HbMP) with narrow size distribution between 800 and 1000 nm, uniform morphology and negative zeta-potential. More than 40% of Hb in the particles was able to carry oxygen over a storage period of 90 days. The results suggest that our new protein submicron particle fabrication technique minimizes the fabrication time and is very efficient and cost-effective.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Substitutos Sanguíneos
/
Hemoglobinas
Limite:
Animals
Idioma:
En
Revista:
Artif Cells Nanomed Biotechnol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha