Pleiotropic effects of a cold shock protein homolog PprM on the proteome of Deinococcus radiodurans.
Biochim Biophys Acta Proteins Proteom
; 1867(2): 98-106, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30389625
ABSTRACT
An extremophile D. radiodurans encodes a non-cold shock inducible cold shock protein homolog DR_0907 (also known as PprM). The DR_0907 ORF was deleted by knockout mutagenesis and the resultant deletion mutant (ΔpprM D. radiodurans) displayed growth defect as well as gamma-radiation sensitivity (D10 valuesâ¯=â¯ΔpprM D. radiodurans 12.1â¯kGy versus wild type (WT) D. radiodurans 14â¯kGy). 2D gel based comparative proteomics revealed a comparable induction of DNA repair proteins in ΔpprM D. radiodurans and WT D. radiodurans recovering from 5â¯kGy gamma irradiation (60Co gamma source, dose rate 2â¯kGy/h), suggesting that pprM does not cause radiation sensitivity through modulation of DdrO-regulated DNA repair genes. However, deletion of pprM did result in repression of several proteins that belonged to vital housekeeping pathways such as metabolism and protein homeostasis that might contribute to slow growth phenotype. These deficiencies intrinsic to ΔpprM D. radiodurans might also contribute to its radiation sensitivity.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Deinococcus
/
Proteínas e Peptídeos de Choque Frio
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Índia