Exosomal lncRNAATB activates astrocytes that promote glioma cell invasion.
Int J Oncol
; 54(2): 713-721, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30483768
ABSTRACT
Glioma invasion is a main cause of a poor prognosis and relapse in patients suffering from the disease. However, the molecular mechanisms responsible for glioma cell invasion remain poorly understood. In this study, the characteristics of exosomes were identified using electron microscope (TEM), and western blot analysis. The potential mechanism of long noncoding RNA (lncRNA) activated by TGFß (lncRNAATB) was demonstrated using luciferase reporter assays and RNA immunoprecipitation. We found that glioma cellderived exosomes promoted the activation of astrocytes and had the ability to shuttle long noncoding RNA (lncRNA) activated by TGFß (lncRNAATB) to astrocytes. More importantly, lncRNAATB activated astrocytes through the suppression of microRNA (miRNA or miR)2043p in an Argonaute 2 (Ago2)dependent manner. Furthermore, astrocytes activated by lncRNAATB in turn promoted the migration and invasion of glioma cells. Taken together, the findings of this study suggest that lncRNAATB may play an important role in modulating glioma microenvironment through exosomes. Thus, a better understanding of this process may provide implications for the prevention of highly invasive glioma.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta
/
Exossomos
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RNA Longo não Codificante
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Glioma
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Int J Oncol
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2019
Tipo de documento:
Article