Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients.
Arch Oral Biol
; 98: 148-155, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30496935
OBJECTIVE: In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown. MATERIALS AND METHODS: Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides. RESULTS: The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes. CONCLUSIONS: The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Glândulas Salivares
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Proteínas e Peptídeos Salivares
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Síndrome da Ardência Bucal
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Proteoma
/
Proteômica
Tipo de estudo:
Qualitative_research
Limite:
Aged
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Female
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Humans
/
Middle aged
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
2019
Tipo de documento:
Article
País de publicação:
Reino Unido