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Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients.
Cabras, Tiziana; Manconi, Barbara; Castagnola, Massimo; Sanna, Maria Teresa; Arba, Morena; Acharya, Shikha; Ekström, Jörgen; Carlén, Anette; Messana, Irene.
Afiliação
  • Cabras T; Department of Life and Environmental Sciences, Biomedical Section, University of Cagliari, 09042, Monserrato, CA, Italy. Electronic address: tcabras@unica.it.
  • Manconi B; Department of Life and Environmental Sciences, Biomedical Section, University of Cagliari, 09042, Monserrato, CA, Italy.
  • Castagnola M; Institute of Chemistry of the Molecular Recognition - CNR, L.go F. Vito 1, 00168, Rome, Italy; Institute of Biochemistry and Clinical Biochemistry, Università Cattolica del Sacro Cuore and/or Department of Laboratory Diagnostic and Infectious Diseases, Fondazione Policlinico Universitario Agostino G
  • Sanna MT; Department of Life and Environmental Sciences, Biomedical Section, University of Cagliari, 09042, Monserrato, CA, Italy.
  • Arba M; Department of Life and Environmental Sciences, Biomedical Section, University of Cagliari, 09042, Monserrato, CA, Italy.
  • Acharya S; Department of Oral Microbiology and Immunology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Box 431, SE-405 30 Göteborg, Sweden.
  • Ekström J; Department of Pharmacology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Box 431, SE-405 30 Göteborg, Sweden.
  • Carlén A; Department of Oral Microbiology and Immunology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Box 431, SE-405 30 Göteborg, Sweden.
  • Messana I; Institute of Chemistry of the Molecular Recognition - CNR, L.go F. Vito 1, 00168, Rome, Italy.
Arch Oral Biol ; 98: 148-155, 2019 Feb.
Article em En | MEDLINE | ID: mdl-30496935
OBJECTIVE: In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown. MATERIALS AND METHODS: Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides. RESULTS: The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes. CONCLUSIONS: The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Glândulas Salivares / Proteínas e Peptídeos Salivares / Síndrome da Ardência Bucal / Proteoma / Proteômica Tipo de estudo: Qualitative_research Limite: Aged / Female / Humans / Middle aged Idioma: En Revista: Arch Oral Biol Ano de publicação: 2019 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saliva / Glândulas Salivares / Proteínas e Peptídeos Salivares / Síndrome da Ardência Bucal / Proteoma / Proteômica Tipo de estudo: Qualitative_research Limite: Aged / Female / Humans / Middle aged Idioma: En Revista: Arch Oral Biol Ano de publicação: 2019 Tipo de documento: Article País de publicação: Reino Unido