Network proteomics of human dermal wound healing.
Physiol Meas
; 39(12): 124002, 2018 12 07.
Article
em En
| MEDLINE
| ID: mdl-30524050
ABSTRACT
OBJECTIVE:
The healing of wounds is critical in protecting the human body against environmental factors. The mechanisms involving protein expression during this complex physiological process have not been fully elucidated.APPROACH:
Here, we use reverse-phase protein microarrays (RPPA) involving 94 phosphoproteins to study tissue samples from tubes implanted in healing dermal wounds in seven human subjects tracked over two weeks. We compare the proteomic profiles to proteomes of controls obtained from skin biopsies from the same subjects. MAINRESULTS:
Compared to previous proteomic studies of wound healing, our approach focuses on wound tissue instead of wound fluid, and has the sensitivity to go beyond measuring only highly abundant proteins. To study the temporal dynamics of networks involved in wound healing, we applied two network analysis methods that integrate the experimental results with prior knowledge about protein-protein physical and regulatory interactions, as well as higher-level biological processes and associated pathways.SIGNIFICANCE:
We uncovered densely connected networks of proteins that are up- or down-regulated during human wound healing, as well as their relationships to microRNAs and to proteins outside of our set of targets that we measured with proteomic microarrays.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Fenômenos Fisiológicos da Pele
/
Cicatrização
/
Proteômica
Limite:
Humans
Idioma:
En
Revista:
Physiol Meas
Assunto da revista:
BIOFISICA
/
ENGENHARIA BIOMEDICA
/
FISIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos