Downregulation of miR2245p in prostate cancer and its relevant molecular mechanism via TCGA, GEO database and in silico analyses.
Oncol Rep
; 40(6): 3171-3188, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30542718
The function of the expression of microRNA (miR)2245p in prostate adenocarcinoma (PCa) remains to be elucidated, therefore, the present study aimed to investigate the clinical significance and potential molecular mechanism of miR2245p in PCa. Data on the expression of miR2245p in PCa were extracted from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), ArrayExpress and previous literature, and metaanalyses with standardized mean difference (SMD) and summary receiver operating characteristic (sROC) methods were performed for statistical analyses. The prospective target genes of miR2245p were collected by overlapping the differentially expressed mRNAs in TCGA and GEO, and target genes predicted by miRWalk2.0. Subsequently, in silico analysis was performed to examine the associated pathways of miR2245p in PCa. The expression of miR2245p was markedly lower in PCa; the overall SMD was 0.562, and overall sROC area under the curve was 0.80. In addition, Kyoto Encyclopedia of Genes and Genomes analysis revealed that the prospective target genes of miR2245p were largely enriched in the amino sugar and nucleotide sugar metabolism signaling pathway, and three genes [UDPNacetylglucosamine pyrophosphorylase 1 (UAP1), hexokinase 2 (HK2) and chitinase 1 (CHIT1)] enriched in this pathway showed higher expression (P<0.05). In addition, key genes in the proteinprotein interaction network analysis [DNA topoisomerase 2α (TOP2A), ATP citrate lyase (ACLY) and ribonucleotide reductase regulatory subunit M2 (RRM2)] exhibited significantly increased expression (P<0.05). The results suggested that the downregulated expression of miR2245p may be associated with the clinical progression and prognosis of PCa. Furthermore, miR2245p likely exerts its effects by targeting genes, including UAP1, HK2, CHIT1, TOP2A, ACLY and RRM2. However, in vivo and in vitro experiments are required to confirm these findings.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
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Adenocarcinoma
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Regulação Neoplásica da Expressão Gênica
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MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Humans
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Male
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2018
Tipo de documento:
Article
País de publicação:
Grécia