Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation.
Viruses
; 10(12)2018 12 19.
Article
em En
| MEDLINE
| ID: mdl-30572664
ABSTRACT
Influenza A virus (IAV) infections remain a major human health threat. IAV has enormous genetic plasticity and can rapidly escape virus-targeted anti-viral strategies. Thus, there is increasing interest to identify host proteins and processes the virus requires for replication and maturation. The IAV non-structural protein 1 (NS1) is a critical multifunctional protein that is expressed to high levels in infected cells. Host proteins that interact with NS1 may serve as ideal targets for attenuating IAV replication. We previously developed and characterized broadly cross-reactive anti-NS1 monoclonal antibodies. For the current study, we used these mAbs to co-immunoprecipitate native IAV NS1 and interacting host proteins; 183 proteins were consistently identified in this NS1 interactome study, 124 of which have not been previously reported. RNAi screens identified 11 NS1-interacting host factors as vital for IAV replication. Knocking down one of these, nuclear mitotic apparatus protein 1 (NUMA1), dramatically reduced IAV replication. IAV genomic transcription and translation were not inhibited but transport of viral structural proteins to the cell membrane was hindered during maturation steps in NUMA1 knockdown (KD) cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Proteínas não Estruturais Virais
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Montagem de Vírus
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Antígenos Nucleares
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Proteínas Associadas à Matriz Nuclear
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Interações Hospedeiro-Patógeno
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Viruses
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Canadá
País de publicação:
CH
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SUIZA
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SUÍÇA
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SWITZERLAND