[The selection of system of detection for multiplex dot-immune analysis of antibodies].

ABSTRACT

The comparative evaluation was carried out concerning systems of detection using conjugates on the basis of peroxidase, alkaline phosphatase, colloid gold and system of amplification "Super-CARD" for multiplex dot-immune assay of antibodies. It is established that sensitivity of system of detection with colloid gold is approximately 8 times higher than with amplification "SuperCARD"; 30 times higher than with conjugate of alkaline phosphatase and 250 times higher than with peroxidase conjugate. The immunesols of gold maintain limit of detection of human IgG 10 pg with dynamic range of signal alteration from 5 ng to10 pg overlapping the range of concentrations of specific IgG in case of typical natural immune response. The most specific results provides conjugate of colloid gold with monoclonal antibodies to human IgG.