Identification and Analysis of VTC2 Homologs Encoding the Key Enzyme of L-Ascorbic Acid Biosynthesis in Tomato Species (Solanum Section of Lycopersicon).
Dokl Biochem Biophys
; 483(1): 374-378, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30607742
ABSTRACT
The complete nucleotide sequences of 11 wild and cultivated tomato GDP-L-galactose-phosphorylase- 1 homologs (VTC2) were identified and characterized. The overall level of nucleotide variability was 9.19%. Totally 25 out of 54 cDNA SNPs were non-synonymous and resulted in amino acid substitutions. In the group of green-fruited tomato species, more SNPs were detected than in the group of red-fruited species, but the percentage of non-synonymous substitutions in red-fruited tomatoes was significantly higher (66.7% vs. 37.5%). In the translated amino acid sequences, the GDF-L-galactose-hexose-1-phosphate-guanyltransferase domain and the highly conserved HIT-motif were identified and a conserved motif specific for VTC2 of Solanoideae species was detected.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Ácido Ascórbico
/
Monoéster Fosfórico Hidrolases
/
Solanum lycopersicum
/
Polimorfismo de Nucleotídeo Único
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Dokl Biochem Biophys
Assunto da revista:
BIOFISICA
/
BIOQUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Federação Russa