Thiopurine intolerance-causing mutations in NUDT15 induce temperature-dependent destabilization of the catalytic site.
Biochim Biophys Acta Proteins Proteom
; 1867(4): 376-381, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30639426
ABSTRACT
Germline mutations in NUDT15 cause thiopurine intolerance during treatment of leukemia or autoimmune diseases. Previously, it has been shown that the mutations affect the enzymatic activity of the NUDT15 hydrolase due to decreased protein stability in vivo. Here we provide structural insights into protein destabilization in R139C and V18I mutants using thermolysin-based proteolysis and H/D exchange followed by mass spectrometry. Both mutants exhibited destabilization of the catalytic site, which was more pronounced at higher temperature. This structural perturbation is shared by the mutations despite their different positions within the protein structure. Reaction products of NUDT15 reverted these conformational abnormalities, demonstrating the importance of ligands for stabilization of a native state of the mutants. This study shows the action of pharmacogenetic variants in NUDT15 in a context of protein structure, which might open novel directions in personalized chemotherapy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pirofosfatases
/
Nucleotídeos de Desoxiguanina
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
República Tcheca