Bioassay for monitoring the anti-aging effect of cord blood treatment.
Theranostics
; 9(1): 1-10, 2019.
Article
em En
| MEDLINE
| ID: mdl-30662549
ABSTRACT
Background:
Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed an impressive potential to slow age-associated degradation of neuronal and cognitive functions. Translating such findings to clinical realities, however, requires effective ways for assessing treatment efficacy; ideal methods should be minimally invasive, amenable for serial assays, cost-effective, and quantitative.Methods:
We developed a new biosensor approach to monitor anti-aging therapy. We advanced two key sensor components i) a blood-borne metabolite was identified as a surrogate aging-marker; and ii) a compact and cost-effective assay system was developed for on-site applications. We treated aged mice either with human umbilical cord plasma or saline; unbiased metabolite profiling on mouse plasma revealed arachidonic acid (AA) as a potent indicator associated with anti-aging effect. We next implemented a competitive magneto-electrochemical sensor (cMES) optimized for AA detection directly from plasma. The developed platform could detect AA directly from small volumes of plasma (0.5 µL) within 1.5 hour.Results:
cMES assays confirmed a strong correlation between AA levels and anti-aging effect AA levels, while decreasing with aging, increased in the plasma-treated aged mice which also showed improved learning and memory performance.Conclusions:
The cMES platform will empower both pre- and clinical anti-aging research by enabling minimally invasive, longitudinal treatment surveillance; these capacities will accelerate the development of anti-aging therapies, improving the quality of individual lives.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfusão de Sangue
/
Envelhecimento
/
Técnicas Biossensoriais
/
Monitoramento de Medicamentos
/
Ácido Araquidônico
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Metabolômica
/
Sangue Fetal
Tipo de estudo:
Observational_studies
/
Prognostic_studies
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Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Theranostics
Ano de publicação:
2019
Tipo de documento:
Article