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Genome sequence of Malania oleifera, a tree with great value for nervonic acid production.
Xu, Chao-Qun; Liu, Hui; Zhou, Shan-Shan; Zhang, Dong-Xu; Zhao, Wei; Wang, Sihai; Chen, Fu; Sun, Yan-Qiang; Nie, Shuai; Jia, Kai-Hua; Jiao, Si-Qian; Zhang, Ren-Gang; Yun, Quan-Zheng; Guan, Wenbin; Wang, Xuewen; Gao, Qiong; Bennetzen, Jeffrey L; Maghuly, Fatemeh; Porth, Ilga; Van de Peer, Yves; Wang, Xiao-Ru; Ma, Yongpeng; Mao, Jian-Feng.
Afiliação
  • Xu CQ; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Liu H; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Zhou SS; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Zhang DX; College of Life Science, Datong University, Datong, Shanxi, 037009, China.
  • Zhao W; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Wang S; Yunnan Key Laboratory of Forest Plant Cultivation and Utilization, State Forestry Administration Key Laboratory of Yunnan Rare and Endangered Species Conservation and Propagation, Yunnan Academy of Forestry, Kunming, Yunnan, 650201, China.
  • Chen F; The Camellia Institute, Yunnan Academy of Forestry, Guangnan, Yunnan, 663300, China.
  • Sun YQ; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Nie S; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Jia KH; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Jiao SQ; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Zhang RG; Beijing Ori-Gene Science and Technology Co. Ltd, Beijing, 102206, China.
  • Yun QZ; Beijing Ori-Gene Science and Technology Co. Ltd, Beijing, 102206, China.
  • Guan W; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Wang X; The Camellia Institute, Yunnan Academy of Forestry, Guangnan, Yunnan, 663300, China.
  • Gao Q; Department of Genetics, University of Georgia, Athens, GA 30602, USA.
  • Bennetzen JL; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, School of Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Maghuly F; The Camellia Institute, Yunnan Academy of Forestry, Guangnan, Yunnan, 663300, China.
  • Porth I; Department of Genetics, University of Georgia, Athens, GA 30602, USA.
  • Van de Peer Y; Plant Biotechnology Unit (PBU), Dept. Biotechnology, BOKU-VIBT, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna 1190, Austria.
  • Wang XR; Département des sciences du bois et de la forêt, 1030, Avenue de la Médecine, Université Laval, Québec (Québec) G1V 0A6, Canada.
  • Ma Y; Institute for System and Integrated Biology, Pavillon Charles-Eugène-Marchand, 1030, Avenue de la Médecine, Université Laval, Québec (Québec) G1V 0A6, Canada.
  • Mao JF; Centre d'Étude de la Forêt, 1030, Avenue de la Médecine, Université Laval, Québec (Québec) G1V 0A6, Canada.
Gigascience ; 8(2)2019 02 01.
Article em En | MEDLINE | ID: mdl-30689848
ABSTRACT

BACKGROUND:

Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree's seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools.

FINDINGS:

We generated 51 Gb and 135 Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10× Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51 Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10× Genomics reads. Identified repeats constituted ∼82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail.

CONCLUSIONS:

Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Genoma de Planta / Olacaceae / Sequenciamento Completo do Genoma Tipo de estudo: Prognostic_studies Idioma: En Revista: Gigascience Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Genoma de Planta / Olacaceae / Sequenciamento Completo do Genoma Tipo de estudo: Prognostic_studies Idioma: En Revista: Gigascience Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China