The Pathometabolism of Legionella Studied by Isotopologue Profiling.
Methods Mol Biol
; 1921: 21-44, 2019.
Article
em En
| MEDLINE
| ID: mdl-30694483
ABSTRACT
Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general 13C-labelled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Legionelose
/
Legionella
/
Isótopos de Carbono
/
Metabolismo Energético
/
Redes e Vias Metabólicas
/
Metaboloma
/
Metabolômica
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha