Detection of Histone Modifications Associated with miRNAs.
Methods Mol Biol
; 1932: 347-357, 2019.
Article
em En
| MEDLINE
| ID: mdl-30701512
ABSTRACT
The posttranslational modifications of histones and miRNAs are key epigenetic mechanisms participating in the development, growth, and reproduction of plants. Recently, coordination between these two mechanisms has been demonstrated; each mechanism can be controlled by the other for the regulation of several biological processes. For example, the acetylation of histone H3, a key modification for chromatin remodeling and gene activation, has been linked to the actions of miRNA. In this work, we describe a method for the isolation and immunodetection of two posttranslational modifications in the residues of lysine 9 and 27 of H3, which have been associated with long miRNAs in plants.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
RNA de Plantas
/
MicroRNAs
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
México