Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers.

Lu, Hung-Hsun; Huang, Cheng-Hung; Shiue, Ting-Yun; Wang, Fu-Sheng; Chang, Ko-Kai; Chen, Yunching; Peng, Chi-How

Lu, Hung-Hsun; Huang, Cheng-Hung; Shiue, Ting-Yun; Wang, Fu-Sheng; Chang, Ko-Kai; Chen, Yunching; Peng, Chi-How.

Afiliação

Lu HH; Department of Chemistry , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan . Email: chpeng@mx.nthu.edu.tw.
Huang CH; Department of Chemistry , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan . Email: chpeng@mx.nthu.edu.tw.
Shiue TY; Institute of Biomedical Engineering , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan.
Wang FS; Department of Chemistry , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan . Email: chpeng@mx.nthu.edu.tw.
Chang KK; Department of Chemistry , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan . Email: chpeng@mx.nthu.edu.tw.
Chen Y; Institute of Biomedical Engineering , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan.
Peng CH; Department of Chemistry , Frontier Research Center on Fundamental and Applied Sciences of Matters , National Tsing Hua University , 101, Sec 2, Kuang-Fu Rd. , Hsinchu 30013 , Taiwan . Email: chpeng@mx.nthu.edu.tw.

Chem Sci ; 10(1): 284-292, 2019 Jan 07.

Article em En | MEDLINE | ID: mdl-30713638

ABSTRACT

ABSTRACT

Triblock copolymer of poly(ethylene glycol)-b-poly(2-dimethylaminoethyl methacrylate)-b-poly(pyrenylmethyl methacrylate) (PEG-b-PDMAEMA-b-PPy) has been developed for use as an ideal gene delivery system, which showed both high stability under physiological conditions and efficient gene release in a mimetic cancer environment. The siRNA release from this system without external stimulation was 16% in 1 h and then remained steady. However, the photo-triggered siRNA release was 78% within 1 h and was higher than 91% after 24 h. The remarkable contrast between the stability and release efficiency of these siRNA-condensed micelleplexes before and after photo-irradiation has been rationalized by the light- and pH-induced structural transitions of the triblock copolymer micelles. The negligible cytotoxicity, high cellular uptake efficiency, and remarkable knockdown efficiency shown in in vitro tests further revealed the promising potential of these triblock copolymer micelleplexes for use in stimuli-responsive gene therapy.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Chem Sci Ano de publicação: 2019 Tipo de documento: Article