Investigating proteome and transcriptome response of Cryptococcus podzolicus Y3 to citrinin and the mechanisms involved in its degradation.
Food Chem
; 283: 345-352, 2019 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-30722882
ABSTRACT
Citrinin (CIT) contamination has been reported in agricultural foods and is known to be nephrotoxic to human and animals. In the present study, the proteomes and transcriptomes of C. podzolicus Y3 treated with or without 10⯵g/mL CIT were compared by two-dimensional electrophoresis (2-DE) and RNA sequencing, respectively. The proteomics results showed that there were 23 differentially expressed proteins (DEPs), 8 DEPs were up-regulated and 15 DEPs were significantly down-regulated. Transcriptomic analysis showed that 1208 genes were differentially expressed, 551 (43.05%) DEGs were up regulated and 657 (56.95%) were down-regulated. These results showed that the CIT treatment caused DNA damage, oxidative stress and cell apoptosis in C. podzolicus Y3. CIT treatment also activated the defense response (DNA repair and drug resistance biological process, antioxidative activity and TCA cycle) as well as drug metabolism (synthesize the CIT-degrading enzymes) in yeast cells to respond to CIT stress and degrade CIT.
Palavras-chave
Citrinin; Citrinin (PubChem CID: 54680783); Cryptococcus podzolicus; Defense response; Degradation mechanism; Dithiothreitol (PubChem CID: 446094); EDTA (PubChem CID: 6049); Glycerol (PubChem CID: 753); Iodoacetamide (PubChem CID: 3727); PMSF (PubChem CID: 4784); Proteome; Thiourea (PubChem CID: 2723790); Transcriptome; Trichloroacetic acid (PubChem CID: 6421); Tris-HCL (PubChem CID: 93573); Urea (PubChem CID: 1176)
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese em Gel Bidimensional
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Citrinina
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Proteoma
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Cryptococcus
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Transcriptoma
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Antibacterianos
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2019
Tipo de documento:
Article