High-resolution characterization of 12 classical and non-classical HLA loci in Southern Brazilians.
HLA
; 93(2-3): 80-88, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30740929
ABSTRACT
The human leukocyte antigen (HLA) are the most polymorphic genes in the human genome. Because of their importance for antigen recognition, HLA molecules play a central role in host defense and graft rejection upon transplantation. The aim of this study was to characterize allelic diversity of the classical HLA genes HLA-A, -B, -C, -DRA, -DRB1, -DQA1, -DQB1, -DPA1, -DPB1, and the non-classical class I genes HLA-E, -F and -G at high-resolution for a population of predominantly European ancestry from Curitiba, Brazil. Genotyping of 108 individuals was performed by next-generation sequencing on the MiSeq platform and also by Sanger sequencing. The genotype distributions of all loci were in accordance with Hardy-Weinberg equilibrium (P > 0.05) and a total of 202 HLA variants at second field resolution were observed for the 12 loci. The strongest linkage disequilibrium (r2 = 1.0, P < 10-5 ) was observed for the following pairs of alleles HLA-B*420101 ~ HLA-DRB1*030201; HLA-B*140201 ~ HLA-C*080201; B*420101 ~ HLA-C*170101; HLA-DRB1*030101 ~ HLA-DQB1*020101 ~ DRB1*030101 ~ HLA-DQB1*020101; DRB1*130101~ HLA-DQB1*060301 and HLA-DRB1*090102 ~ HLA-DQA1*0302. This is the first study to characterize all 12 HLA genes at high resolution in a single population. On the basis of the allelic frequencies of worldwide populations and principal component analysis, we confirmed the similarity of the study population to European and other Euro-descendant populations.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos de Histocompatibilidade Classe I
/
Loci Gênicos
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
HLA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil