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High rate misidentification of biochemically determined Streptococcus isolates from swine clinical specimens.
Meekhanon, Nattakan; Kaewmongkol, Sarawan; Jirawattanapong, Pichai; Kaminsonsakul, Tanyanant; Kongsoi, Siriporn; Chumsing, Suksan; Okura, Masatoshi; Ueno, Yuichi; Sekizaki, Tsutomu; Takamatsu, Daisuke.
Afiliação
  • Meekhanon N; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
  • Kaewmongkol S; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
  • Jirawattanapong P; Department of Farm Resources and Production Medicines, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
  • Kaminsonsakul T; Kamphaengsaen Veterinary Diagnostic Unit, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
  • Kongsoi S; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
  • Chumsing S; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
  • Okura M; Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
  • Ueno Y; Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
  • Sekizaki T; Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
  • Takamatsu D; Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci ; 81(4): 567-572, 2019 Apr 16.
Article em En | MEDLINE | ID: mdl-30814435
In this study, 22 bacterial isolates from swine necropsy specimens, which were biochemically identified as Streptococcus suis and other Streptococcus species, were re-examined using species-specific PCR for authentic S. suis and 16S rRNA gene sequencing for the verification of the former judge. Identification of S. suis on the basis of biochemical characteristics showed high false-positive (70.6%) and false-negative (60%) rates. The authentic S. suis showed various capsular polysaccharide synthesis gene types, including type 2 that often isolated from human cases. Five of 22 isolates did not even belong to the genus Streptococcus. These results suggested that the misidentification of the causative pathogen in routine veterinary diagnosis could be a substantial obstacle for the control of emerging infectious diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estreptocócicas / Doenças dos Suínos / Streptococcus suis Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: J Vet Med Sci Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Tailândia País de publicação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estreptocócicas / Doenças dos Suínos / Streptococcus suis Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: J Vet Med Sci Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Tailândia País de publicação: Japão