Your browser doesn't support javascript.
loading
Quantification and Localization of Formylated Phloroglucinol Compounds (FPCs) in Eucalyptus Species.
Dos Santos, Bruna Marques; Zibrandtsen, Juliane F S; Gunbilig, Disan; Sørensen, Mette; Cozzi, Federico; Boughton, Berin A; Heskes, Allison Maree; Neilson, Elizabeth Heather Jakobsen.
Afiliação
  • Dos Santos BM; Section for Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Zibrandtsen JFS; VILLUM Center for Plant Plasticity, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Gunbilig D; Section for Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Sørensen M; VILLUM Center for Plant Plasticity, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Cozzi F; Section for Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Boughton BA; VILLUM Center for Plant Plasticity, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Heskes AM; Section for Plant Biochemistry, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Neilson EHJ; VILLUM Center for Plant Plasticity, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Front Plant Sci ; 10: 186, 2019.
Article em En | MEDLINE | ID: mdl-30863416
ABSTRACT
The Eucalyptus genus is a hyper-diverse group of long-lived trees from the Myrtaceae family, consisting of more than 700 species. Eucalyptus are widely distributed across their native Australian landscape and are the most widely planted hardwood forest trees in the world. The ecological and economic success of Eucalyptus trees is due, in part, to their ability to produce a plethora of specialized metabolites, which moderate abiotic and biotic interactions. Formylated phloroglucinol compounds (FPCs) are an important class of specialized metabolites in the Myrtaceae family, particularly abundant in Eucalyptus. FPCs are mono- to tetra-formylated phloroglucinol based derivatives, often with an attached terpene moiety. These compounds provide chemical defense against herbivory and display various bioactivities of pharmaceutical relevance. Despite their ecological and economic importance, and continued improvements into analytical techniques, FPCs have proved challenging to study. Here we present a simple and reliable method for FPCs extraction, identification and quantification by UHPLC-DAD-ESI-Q-TOF-MS/MS. The method was applied to leaf, flower bud, and flower samples of nine different eucalypt species, using a small amount of plant material. Authentic analytical standards were used to provide high resolution mass spectra and fragmentation patterns. A robust method provides opportunities for future investigations into the identification and quantification of FPCs in complex biological samples with high confidence. Furthermore, we present for the first time the tissue-based localization of FPCs in stem, leaf, and flower bud of Eucalyptus species measured by mass spectrometry imaging, providing important information for biosynthetic pathway discovery studies and for understanding the role of those compounds in planta.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Dinamarca

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Dinamarca