miR124 regulates the osteogenic differentiation of bone marrowderived mesenchymal stem cells by targeting Sp7.
Mol Med Rep
; 19(5): 3807-3814, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30896834
ABSTRACT
MicroRNAs (miRNAs) are novel key regulators of cellular differentiation. miR124 has been reported to regulate osteogenic differentiation of bone marrowderived mesenchymal stem cells (BMSCs). However, the specific mechanisms involved have not yet been fully elucidated. The present study aimed to investigate the effect of miR124 on osteogenic differentiation of BMSCs and its underlying mechanisms. In the present study, it was found that alkaline phosphatase (ALP) activity, osteocalcin (OC) secretion, and the protein levels of osterix (Sp7) and runtrelated transcription factor 2 (Runx2) were significantly increased, whereas the expression of miR124 was decreased in a timedependent manner during osteogenic differentiation of BMSCs. Following overexpression of miR124 via transfection of miR124 mimics in BMSCs, Runx2 protein expression and ALP activity were significantly decreased. By contrast, inhibition of miR124 expression led to an increase in ALP activity and Runx2 expression. Sp7 expression was suppressed in BMSCs transfected with miR124 mimics while increased when miR124 expression was inhibited, indicating that miR124 regulates the expression of Sp7. Moreover, a luciferase reporter assay further verified that Sp7 is the direct target of miR124. Finally, the effect of miR124 inhibitor on promoting the differentiation of BMSCs was abolished following treatment with a small interfering RNA targeting Sp7. Taken together, the present study demonstrates that miR124 inhibits the osteogenic differentiation of BMSCs by targeting Sp7.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Diferenciação Celular
/
Regulação da Expressão Gênica
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MicroRNAs
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Células-Tronco Mesenquimais
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Fator de Transcrição Sp7
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2019
Tipo de documento:
Article